Thrombin time (TT) test
The TT test is sensitive to a deficiency of fibrinogen or inhibition of thrombin. It measures the formation of a fibrin clot by the action of thrombin on fibrinogen.
Principle of test
Thrombin is added to citrated plasma at 37°C. The time taken for the mixture to clot is measured and the appearance of the
clot noted.
Reagent
The use of a thrombin time test kit is recommended.
This provides the correct concentration of thrombin to use in the test, i.e. that which gives a clotting time of 12–15 seconds with pooled normal plasma.
Collect venous blood into citrate
anticoagulant and centrifuge to obtain platelet poor plasma as described for the APTT test. Perform the test with as little delay as possible.
See also:
Lablink 