Fungal elements that may be observed are budding yeast, hyphae and pseudohyphae, conidia, thin branching filaments resembling bacteria(fungus like bacteria),granules ,or coccidioides immitis spherules.
Preparation of reagent
Normal saline:
Nacl 0.85 g in 100 mls distilled water.
The procedure of saline mount
1. Place one drop of specimen on a glass slide and add one drop of saline.
2. Place coverslip and observed under low and high power under microscope.
Organism appear refractile, shinny or slightly green. Differentiate yeast cells from RBCs; yeast contain inclusion while others do not.
Look for spores,branching hyphae
See also:
Lablink 