When grouping a donor, use the blood sample collected at the time of blood donation. Test it within 24–36 hours. When grouping a patient, collect 5–7 ml of venous blood into a dry glass tube and label
clearly. When completely clotted, centrifuge the blood at about 1 000 g for 3–5 minutes to separate the cells from the serum. Also collect an EDTA blood sample. Make a 3–5% washed red cell suspension as follows:
- Transfer about 0.5 ml of EDTA blood (or red cells from a donor blood sample) into about 5 ml physiological saline.
- Centrifuge at about 1 000 g for 2–3 minutes.
- Discard the supernatant fluid, resuspend the sedimented red cells in a further 5–7 ml saline and centrifuge. Discard the supernatant fluid.
- Make a 3–5% red cell suspension by mixing 1 drop of sedimented cells in 20–25 drops of saline (hold the Pasteur pipette vertically)
See also:
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