● Basic physical examination: To include a check for swollen glands, skin rashes, signs of intravenous drug use or abnormal bleeding (purpura).
● Weight of the person: Persons weighing 45–50 kg or more can safely donate 450 ml of blood.
● Temperature of the person (to exclude any febrile disease e.g. malaria): A donor should not give blood when their temperature is raised.
● Measurement of blood pressure: A donor should not have an abnormally low blood pressure nor a high blood pressure. The upper acceptable limits are a diastolic pressure of 100 mm Hg and systolic pressure of 180 mm Hg. The minimum acceptable blood pressure is 90/50 mm Hg.
● Pulse rate of the person: The pulse rate should be regular and less than 100 beats/minute (counting for at least 30 seconds).
● Test to check for anaemia: For example, measurement of haemoglobin or PCV or an estimate of haemoglobin level using the Haemoglobin Colour Scale. In most countries persons are accepted as blood donors with a haemoglobin of 120 g/l (12 g/dl) or more and haematocrit of 380 g/l (38%) or more. In some countries the lower limit for men is set at 130 g/l (13 g/dl). Higher haemoglobin levels will be
required at high altitudes.