(Courtesy of Diagen Thrombin Test Time Kit. Test )
Test the patient’s plasma and
control plasma in duplicate.
- Pipette 200 l (0.2 ml) of plasma into a small glass tube. Incubate at 37°C for 1–2 minutes.
- Add (0.1 ml) of thrombin, mix and start the stop-watch. Hold the tube in the water bath and tilt the mixture back and forth, looking for clot formation. When a clot forms, stop the stopwatch and record the time in seconds.
- Report the patient’s TT (average of the duplicate tests) providing the TT of the control plasma is satisfactory.
What are the normal ranges for TT?
12–15 seconds
What are causes of prolonged TT?
- DIC and other conditions which produce a low fibrinogen level
- Abnormal fibrinogen
- Treatment with heparin
- Liver failure
- Presence of inhibitors of thrombin such as FDPs
See also:
Lablink 