Sabouraud’s agar is sufficient for the recovery of dermatophytes from cutaneous samples and yeasts from genital cultures.
It is nutritionally poor with acidic pH (5.6).
Peptone (Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue) provide the nitrogen and vitamin source required for organism growth in SDA. Dextrose is added as the energy and carbon source. Agar is the solidifying agent.
Not recommended as a primary isolation medium because it is insufficiently rich to recover certain fastidious pathogenic species, particularly most of the dimorphic fungi.
It does not promote conidiation of filamentous fungi.
Sabouraud’s dextrose agar (2%) is most useful as a medium for the subculture of fungi recovered on enriched medium to enhance typical sporulation and provide the more characteristic colony morphology.
Identification of fungi is performed by observing various aspects of colony morphology, characteristic microscopic structures, rate of growth, media which supports the organism’s growth, and source of specimen.
Yeasts will grow as creamy to white colonies. Molds will grow as filamentous colonies of various colors.
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