COLORIMETRIC GLUCOSE METHOD

COLORIMETRIC GLUCOSE METHOD
The glucose-oxidase enzymatic method is recommended because it is specific for glucose. A protein precipitation stage is included because this removes substances such as urate which may be present in blood samples in sufficient concentration
to interfere in the final stage of the reaction.

Principle of glucose oxidase-peroxidase
method

Glucose oxidase (GOD) catalyzes the oxidation of glucose to give hydrogen peroxide (H2O2) and gluconic acid. In the presence of the enzyme peroxidase (POD), the hydrogen peroxide is broken
down and the oxygen released reacts with 4- aminophenazone (4-aminoantipyrine) and phenol to give a pink colour.
The absorbance of the colour produced is measured in a colorimeter using a green filter 520 nm or in a spectrophotometer at
515 nm.

See also:

• GTT TEST
• BLOOD GLUCOSE TEST