HOW TO MAKE A THIN BLOOD FILM


1. Make a blood spreader from a slide which has ground glass polished sides

2. Place a drop of blood on the end of a clean dry slide too large (if too large, use a drop from the excess blood to make the film).

3. Using a clean smooth edged spreader, draw the spreader back to touch the drop of blood and allow the blood to extend along the edge of the spreader. Holding the spreader at an angle of about 30, spread the drop of blood to make a film about 40–50 mm in length (two thirds of the slide)

4. Wipe clean the end of the spreader.

5. Immediately air dry the film by waving the slide back and forth. Protect the dried film from dust and insects.

When not using a frosted ended slide,
write the patient’s name and number on the dried blood at the top or along the side of the film using a lead pencil.

6. When completely dry and within a few minutes of making the blood film, fix it in absolute methanol

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