1.Take an inoculating needle, usually a thin needle or wire at the end of a long pencil-like handle, and heat it in an alcohol or gas flame until it glows bright red
2. Allow the needle to cool for about 15 seconds. (A hot needle will kill the mould that is to be transferred).
3. Open the Petri dish containing the culture just wide enough to allow entry of the needle.
4. With the heat-sterilized needle, cut out a small portion of the colony margin. Hyphal tip transfers work best as they are usually the most active parts of the culture; in addition, transfers from the heavily sporulating central portions will result in spores being spread into the air. Especially in medical work, hyphal tip transfers are essential. The excised colony margin should be only about 1 mm square.
5. Transfer the square of colony margin to the sterile plate, making sure that the lid is opened only wide enough to admit the needle and make the transfer. Place the block at the centre, withdraw the needle and flame it until it is red hot, to kill all adhering spores and hyphae.
6. Close the lid; label the plate with a marking pen, including name of culture and date. We usually wrap a thin strip of paraffin film around the sides of the plate to cover the opening, but this is not absolutely necessary; just a couple of pieces of masking tape to hold the lid down will do.
7. Leave the culture to grow in a protected place that has as little air movement as possible.
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